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A Novel Hybrid Photoacoustic-Fluorescent contrast Agent to Track Aqueous Humour Pathways in Vivo

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posted on 23.05.2021, 11:08 by Kirsten Cardinell
Contrast agents are frequently used in photoacoustic imaging (PAI) to study the lymphatic system. However, there is a lack of standardized approaches to evaluate agentsfor specific applications. Furthermore, validation of PAI results through post-mortem fluorescence imaging is often required. To address this, hybrid modality contrast agents would be advantageous. Using unbiased characterization experiments and analyses three commercial NIR dyes are compared: IRDye QC-1, IRDye800CW, and CF770 for the minimum detectable concentration, linear range, and stability of PA signal with prolonged laser exposure. QC-1 had the most desirable properties for quantitative lymphatic PAI specifically for glaucoma treatment testing. Therefore a hybrid tracer was developed and QC-1 alongside fluorescent dye BODIPY was labelled to bovine serum albumin (QBB). QBB tracer showed nearly identical PA properties to free QC-1 dye and maintained a strong fluorescent signal after laser exposure. The QBB tracer shows promise as a hybrid contrast agent for in vivo lymphatic PAI.

History

Language

eng

Degree

Master of Science

Program

Biomedical Physics

Granting Institution

Ryerson University

LAC Thesis Type

Thesis

Usage metrics

Biomedical Physics (Theses)

Keywords

Exports