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Examining Plasmid Transfer of pJP4 in a Mixed Community Biofilm Formed in a Microcosm Simulating a Porous Aquifer

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thesis
posted on 22.05.2021, 17:18 by Floriana Postelnik
In this work, a lab-scale microcosm was designed and used to simulate a porous groundwater aquifer. A minimal media acted as the non-selective pressure for plasmid transfer, while a minimal media with gentamicin acted as the selective pressure. PCR, plate counts and confocal laser scanning microscopy were used to monitor transconjugant and donor persistence in the microcosm. The donor was identified through

History

Language

eng

Degree

Master of Science

Program

Molecular Science

Granting Institution

Ryerson University

LAC Thesis Type

Thesis