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Feasibility Of Establishing An Enzyme-Linked Immunosorbent Assay (ELISA) Laboratory For The Detection Of Dioxins In Economically Marginalized And Developed Countries

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thesis
posted on 23.05.2021, 14:59 by Adrienne K. Lee
Finding a low cost and accessible means of detecting for dioxins in contaminated soil is a necessary step to ensure the health and safety of humans and the environment worldwide. Conventional technologies based on mass spectrometry are expensive and inaccessible. A minimally resourced laboratory and the use of ELISA will be discussed as a feasible, accessible, low cost alternative. The correlation between a minimally resourced laboratory (Ryerson University) and a fully resourced laboratory (Ontario Ministry of the Environment) was strong (n=13, r²=0.888, slope=0.87). To demonstrate the functionality of the minimally resourced laboratory, a supplemental site was characterized using ELISA. Results from the Ryerson and OMOE laboratories produced similar dioxin concentrations of undetectable to 120.26pgTEQg⁻¹ and 32.38 to 163.2pgTEQg⁻¹, respectively. This study illustrates an alternative for evaluating contaminated soil that could serve as a technology transfer for marginalized economies, and provide an accessible form of sample analysis in developed countries.

History

Language

eng

Degree

Master of Applied Science

Program

Environmental Applied Science and Management

Granting Institution

Ryerson University

LAC Thesis Type

Thesis