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Monitoring vascular changes induced by photodynamic therapy using contrast-enhanced micro-computed tomography

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thesis
posted on 24.05.2021, 10:14 by Otilia Cristina Nasui
The aim of this study is to determine if contrast-enhanced micro-computed tomography can be used to non-invasively image the response of vasculature in tumours that have been treated with photodynamic theraphy (PDT). The subjects used were C3H mice with RIF-1 tumour implanted subcutaneously and allowed to grow for 3 weeks prior to treatment. The subjects in this study were divided into PDT treated groups (150 J/cm², 50 J/cm²) and control groups (150 J/cm² light-only, untreated). The contast-enhanced micro-computed tomography imaging procedure consisted of eight-second scans taking place before treatment and up to 24 hours after treatment. The treatment response was evaluated through the ration of blood-to-tumour volume. Significant changes were detected at 8 and 24 hours in the 150 J/cm² PDT group (p< 0.01). Immunohistochemical staining confirmed the effects of each treatment in comparison to the control groups.

History

Language

eng

Degree

Master of Science

Program

Biomedical Physics

Granting Institution

Ryerson University

LAC Thesis Type

Thesis

Thesis Advisor

N. Ford