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Principles and techniques towards successful development of enzyme-linked immunosorbent assay (ELISA) for dioxin analysis

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thesis
posted on 22.05.2021, 16:14 by Wei Zhang
Dioxins are highly toxic, persistent and bio-accumulative compounds. Laboratory detection of dioxins in various environmental matrices is one of the most technically demanding and expensive tasks in analytical chemistry.The cost to analyze a soil sample by conventional gas chromatography-high resolution mass spectrometry (GC-HRMS) is approximately $1,900 USC accordign to the Unisted States Environmental Protection Agency (US EPA) (Billets, 2005). As an alternative, enzyme-linked immunosorbent assay (ELISA) for dioxin analysis has been commercially available for over a decade and recognized as the US EPA Method 3025. However, assay attributes need to be examined, especially at trace level detection. In this study, sources of error in ELISA, such as background contamination and dioxin-like polycholrinated biphenyl (dl-BCB) cross-reactions have been investigated. Quality assurance data on spikes have been reviewed and the recovery was estimated to be 70%. Technical details that are crucial for the performance of dioxin in ELISA were also identified and addressed.

History

Language

eng

Degree

Master of Applied Science

Program

Environmental Applied Science and Management

Granting Institution

Ryerson University

LAC Thesis Type

Thesis

Thesis Advisor

James Li Ching Lo