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Pure-culture and mixed community biofilm responses to carbon-starvation and UV-C exposure /

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posted on 23.05.2021, 15:15 by C Alexia Lane
Biofilms are known to contribute to disease through inherent protective mechanisms and propagation strategies. These multi-cellular systems also play essential roles in numerous environmental processes. The current study investigated the responses of a mixed community biofilm to carbon-starvation, and measured the effects of UV-C on pure-culture biofilms at different stages of maturity by monitoring metabolic and cell yield responses. Carbon dioxide production and biofilm-derived planktonic cell yield were used at the measurement parameters. The mixed community rapidly responded to induced carbon-starvation under continuous flow conditions by remaining metabolically inactive throughout the 96 and 120 h starvation periods, only to promptly return to a metabolically active state upon the reintroduction of carbon. The effects of UV-C on pure-culture biofilms was negligible, with no log activation being achieved, and metabolic activity remaining static.

History

Language

eng

Degree

Master of Applied Science

Program

Environmental Applied Science and Management

Granting Institution

Ryerson University

LAC Thesis Type

Thesis

Thesis Advisor

Gideon M. Wolfaardt