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Structure/Function Analysis of the Hif1 Histone Chaperone in Saccharomyces cerevisiae

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thesis
posted on 22.05.2021, 14:00 by Nora Saud Dannah
Understanding the regulation of chromatin structure is a vital aspect of molecular biology regarding its influences on biological processes such as DNA replication, transcription (gene expression), DNA repair, chromosome segregation and recombination. In the budding yeast Saccharomyces cerevisiae, a histone chaperone called Hif1 has been found in the nuclei as having a functional role in chromatin assembly. Hif1 is a homolog of the human protein NASP that is involved in the maintenance of genome stability. Previously, Hif1 has been shown to physically interact with Hat1, Hat2 and H3/H4 to form the NuB4 complex directly involved in chromatin assembly. A molecular genetic approach was conducted to determine which domain of Hif1 is involved in the interaction with the HAT1 complex.

History

Degree

Master of Science

Program

Molecular Science

Granting Institution

Ryerson University

LAC Thesis Type

Thesis

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